Journal: RSC advances

Article Title: Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cells.

doi: 10.1039/c9ra01072f

Figure Lengend Snippet: Fig. 11 Apoptotic mechanism of HEp-2 cells treated with pcAgNPs. DNA fragmentation of HEp-2 cells exposed to pcAgNPs (a). RT-PCR analysis of p53 expression, leading to apoptotic cell death (b and c); pcAgNP activation of p53, leading to increased catalase expression. HEp-2 cells treated with 15 mg mL1 pcAgNPs for 24 h, showing p53, p21 and catalase expressions assessed using Western blotting analysis. b-actin served as a loading control. Numbers were calculated by quantitative densitometric analysis and indicate the ratio of specific proteins versus b-actin. (d) Data are representative of at least three independent experiments.

Article Snippet: In the western blotting analysis, the following primary antibodies were used: rabbit polyclonal anti-p21 (1 : 100) (cat. no. TA322940, OriGene, USA), mouse monoclonal anti-p53 (1 : 500) (cat. no. TA327920, OriGene, USA), andmouse monoclonal anticatalase (1 : 100) (cat. no. TA502496, OriGene, USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Activation Assay, Western Blot, Control